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硝基化酪氨酸与酪氨酸在结构上相似,它在病理情况下会出现,并在细胞内与微管蛋白结合,从而阻碍微管的正常功能.硝基化酪氨酸在肿瘤中的作用,目前研究甚少.本文利用头颈鳞癌Hep-2细胞株,研究微管蛋白酪氨酸连接酶类似物12(tubulin tyrosine ligase like 12,TTLL12)和硝基化酪氨酸对头颈鳞癌Hep-2生长的影响,通过Western印迹试验和MTT试验发现,随着硝基化酪氨酸的浓度升高,细胞内生成的硝基化酪氨酸微管蛋白含量也增高,同时细胞生长受抑制的程度显著增高;对建立的TTLL12高表达细胞株加入硝基化酪氨酸培养,结果显示,TTLL12高表达细胞株内的硝基化酪氨酸微管蛋白含量明显低于对照组细胞;对照组细胞的生长明显受到抑制,而高表达细胞株的生长无明显改变,两者的细胞生长有显著性差异(P<0.05).本研究结果提示,TTLL12可通过阻碍硝基化酪氨酸与微管蛋白的结合,使头颈鳞癌Hep-2细胞逃避硝基化酪氨酸的打击.对这一调控机制的进一步研究,必将有助于控制肿瘤细胞的生长,为治疗肿瘤寻找到新的治疗靶点.
Nitrotyrosine is structurally similar to tyrosine and appears in pathological conditions and binds to tubulin in the cell, hindering the normal functioning of microtubules. Nitrotyrosine is found in tumors , So far, little is known.In this study, Hep-2 cell lines of head and neck squamous cell carcinoma were used to study the effects of tubulin tyrosine ligase like 12 (TTLL12) and nitrotyrosine on head and neck squamous cell carcinoma The effect of Hep-2 on the growth of Hep-2 cells was examined by western blotting and MTT assay. As the concentration of nitrotyrosine increased, the content of intracellular nitrotyrosine tubulin increased, and cell growth The degree of inhibition was significantly increased; the establishment of TTLL12 high expression of cell lines with nitrotyrosine culture, the results showed that TTLL12 high expression of nitrotyrosine tubulin content was significantly lower than the control group of cells (P <0.05) .This study suggests that TTLL12 can inhibit nitration of tyrosine by inhibiting the growth of cells in the control group, while the growth of the cells with high expression is not significantly changed (P <0.05) The combination of acid and tubulin, head and neck scales Hep-2 cells to escape nitrotyrosine blow. Further study of this regulatory mechanism, it will help control the growth of tumor cells, for the treatment of cancer to find new therapeutic targets.