应用半套式PCR技术从Karp株恙虫病立克次体基因组中扩增出872hpDNA片段.该片段经Pstl消化后,与经PStl酶解的pUC18连接,转化大肠杆菌,电泳初筛得pUCRL3等重组质粒.pUCRL3经PCR及酶切证实含约294hp恙虫病立克次体sta56基因片段,可作为检测恙虫病立克次休的特异核酸探针.“,”From the genome of Rickettsia tsutsugamushi, strain Karp,an 872 --hp fragment of partial sta56 gene was amplified by semi--nested polymerase chain reaction. A 294--hp Pstl fragment deriving from the 872hp one was cloned into pUC18 yielding pUCRL3,the recombinant plasmid was confirmed by PCR using. R.tsutsugamushi specific primer and pUC/M13 forward sequencing primer. The probe can be used to detect. R.tsul-sugamushi DNA by nucleic acid Hybridization.