利用本室设计的引物对我国人丙型肝炎病毒核心蛋白基因进行了cDNA合成和扩增,获得了一长534bp的cDNA片段,定名为Q534。将此片段克隆到pUC18质粒中,并进行了序列分析。结果表明所获得的cDNA克隆位于Chiron序列的320-853位,此序列与原型相应序列在核苷酸和氨基酸水平上的同源性分别为90.9％和97.6％,与日本HCV-J株和BK株相应序列的核苷酸同源性为96.8％和97.0％;氯基酸的同源性为98.2％和98.8％。序列比较结果表明此序列属HCVⅡ组。 The cDNA of HCV core protein gene of human was amplified and synthesized by using the primers designed in our laboratory. A 534 bp cDNA fragment was obtained and named as Q534. This fragment was cloned into pUC18 plasmid and subjected to sequence analysis. The results showed that the obtained cDNA clone was located at the positions 320-853 of Chiron sequence. The homology between the sequence and the corresponding sequence of the prototype was 90.9% and 97.6% respectively at the nucleotide and amino acid levels, The homology of the corresponding sequences of the strains was 96.8% and 97.0%, respectively. The identities of the chlorinated acids were 98.2% and 98.8%. Sequence comparison results show that this sequence is HCV Ⅱ group.