眼球是一个构造复杂的球形体,其体积大,巩膜致密,球内有坚硬的晶状体,液体不易渗透致使制作切片难度较高。关键的难点在两个方面:其一是往往因为组织固定不好而造成球内结构分离的现象,如:视网膜与巩膜分离等。传统的方法主张注射固定(颈动脉注射或眼球内注射)但给取材、固定的过程带 Eye is a complex structure of the spherical body, its volume, scleral dense, hard ball inside the lens, the liquid is not easy to penetrate resulting in the production of high difficulty. The key difficulties in two aspects: First, often because of poor tissue structure caused by the separation of the phenomenon within the ball, such as: the separation of the retina and sclera. The traditional method advocates the injection of fixed (carotid artery injection or intraocular injection) but to the material, the process of fixing