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目的:建立五淋散胶囊中栀子苷和芍药苷的HPLC含量测定方法。方法:采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(20∶80),流速为1.0 mL.min-1,检测波长为235 nm;柱温为30℃;进样量为20μL。结果:栀子苷进样量在0.162~1.296μg范围内线性关系良好(r=0.999 9),平均回收率为101.30%,RSD为0.54%(n=6);芍药苷在0.160~1.280μg范围内线性关系良好(r=0.999 9),平均回收率为99.75%,RSD为1.04%(n=6)。结论:本方法简便、准确、快速、灵敏度高、重现性好,可作为五淋散胶囊中栀子苷和芍药苷的定量控制质量标准。
Objective: To establish a HPLC method for determination of geniposide and paeoniflorin in Wulian powder capsule. Methods: Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was methanol-water (20:80) at a flow rate of 1.0 mL · min- Injection volume of 20μL. Results: The calibration curve of geniposide was linear in the range of 0.162-1.296 μg (r = 0.999 9) with an average recovery of 101.30% and a RSD of 0.54% (n = 6) The linearity was good (r = 0.999 9) with an average recovery of 99.75% and a RSD of 1.04% (n = 6). Conclusion: The method is simple, accurate, rapid, sensitive and reproducible. It can be used as quantitative control standard for geniposide and paeoniflorin in Wulian powder capsule.