论文部分内容阅读
目的 研究极低频电磁场暴露对小鼠脑和肝组织c fosmRNA水平的影响。方法 将小鼠暴露于 5 0Hz、0 .2mT及 5 0Hz、6 .0mT的电磁场中 ,持续 2周或 4周 ;采用竞争性RT PCR方法检测小鼠脑和肝脏c fosmRNA水平。结果 5 0Hz、0 .2mT及 5 0Hz、6 .0mT电磁场暴露 2周后 ,小鼠脑组织c fosmRNA的水平上升为 ( 0 .0 178± 0 .0 0 76 )amol 12 0ngcDNA和 ( 0 .0 0 92± 0 .0 0 4 2 )amol 12 0ngcDNA ,与对照组 [( 0 .0 0 12± 0 .0 0 0 5 )amol 12 0ngcDNA]比较 ,差异有显著性 (P <0 .0 5 ) ;肝组织c fosmRNA的水平上升为 ( 0 .0 117± 0 .0 0 5 5 )amol 12 0ngcDNA和 ( 0 .0 14 8± 0 .0 16 2 )amol 12 0ngcDNA ,与对照组[( 0 .0 0 0 5± 0 .0 0 0 5 )amol 12 0ngcDNA]比较 ,差异有显著性 (P <0 .0 5 )。 0 .2mT和 6 .0mT电磁场暴露4周后 ,小鼠脑组织c fosmRNA的水平上升为 ( 0 .0 10 0± 0 .0 0 5 4 )amol 12 0ngcDNA和 ( 0 .0 198±0 .0 0 79)amol 12 0ngcDNA ,与对照组 [( 0 .0 0 15± 0 .0 0 0 8)amol 12 0ngcDNA]比较 ,差异有显著性 (P <0 .0 5 ) ;肝组织c fosmRNA的水平分别上升为 ( 0 .0 173± 0 .0 12 2 )amol 12 0ngcDNA和 ( 0 .0 133±0 .0 0 90 )amol 12 0ngcDNA ,而对照组无表达。结论 5 0Hz电磁?
Objective To study the effect of ultra-low frequency electromagnetic field exposure on c fos mRNA levels in brain and liver of mice. Methods Mice were exposed to 50Hz, 0.2mT and 50Hz, 6. 0mT electromagnetic fields for 2 weeks or 4 weeks. The c fos mRNA levels in brain and liver of mice were detected by competitive RT-PCR. Results The levels of c fos mRNA in mouse brain tissue after 5 0 Hz, 0.2 mT and 50 Hz, 6. 0 mT electromagnetic fields were increased to (0 .0178.00 ± 0.076) amol 12 0 ng cDNA and (0 .0 0 92 ± 0. 0 4 2) amol 12 0ngcDNA, compared with the control group [(0 0 12 ± 0. 0 0 0 5) amol 12 0ngcDNA], the difference was significant (P <0.05) ; The level of c fos mRNA in liver tissue increased to (0 .0 117 ± 0. 0 055) amol 12 0ngcDNA and (0. 14 8 ± 0. 0 16 2) amol 12 0ngcDNA, compared with the control group [(0. 0 0 0 ± 0. 0 0 0 5) amol 12 0ng cDNA], the difference was significant (P <0. 05). After 4 weeks of exposure to 0.2 mT and 0.6 mT EMF, the level of c fos mRNA in mouse brain tissue increased to (0 .10 0 ± 0 .0 05 4) amol 12 0 ng cDNA and (0 .0190 ± 0 .0 0 79) amol 12 0 ng cDNA, compared with the control group [(0 .015 ± 0 .0 0 0 8) amol12 0ngcDNA], the difference was significant (P <0. 05); liver tissue c fosmRNA level (0 .0 173 ± 0 .0 12 2) amol 12 0ngcDNA and (0 .013 ± 0. 0 0 90) amol 12 0ngcDNA, respectively, but not in the control group. Conclusion 5 0Hz electromagnetic?