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目的通过建立宣威地区非小细胞肺癌的裸鼠移植瘤模型,探讨阻断Wnt-1信号传导通路对非小细胞肺癌的抑制作用。方法将21只重量16~18 g的荷瘤小鼠分为三组:空白组(n=7)、对照组(n=7)和实验组(n=7)。空白组注入生理盐水,对照组注入多西他赛,实验组注入Wnt-1抗体。待6次注射干预后,处死所有裸鼠获取肿瘤标本,比较其体积大小,比较空白组、对照组及实验组对肿瘤的抑瘤率。结果肿瘤体积在第21天和27天时空白组分别与实验组、对照组的差异有统计学意义(P=0.002,P=0.000)。体内实验结果显示,多西他赛和Wnt-1抗体对非小细胞肺癌均有抑制作用,多西他赛的抑制作用更强。结论阻断Wnt信号通路对肿瘤的生长有抑制作用,多西他赛和Wnt-1抗体对非小细胞肺癌的抑制有积极的效果。
OBJECTIVE: To establish a nude mouse xenograft model of non-small cell lung cancer in Xuanwei district to investigate the inhibitory effect of Wnt-1 signaling pathway on non-small cell lung cancer. Methods Twenty-one tumor-bearing mice weighing 16-18 g were divided into three groups: blank group (n = 7), control group (n = 7) and experimental group (n = 7). The control group was injected docetaxel into the blank group, and the Wnt-1 antibody was injected into the experimental group. After 6 injection injections, all the nude mice were sacrificed to obtain the tumor samples, and their tumor size was compared. The tumor inhibition rates of the blank group, the control group and the experimental group were compared. Results There was a significant difference in the tumor volume between the control group and the control group on day 21 and 27 (P = 0.002, P = 0.000). In vivo experiments showed that docetaxel and Wnt-1 antibody had inhibitory effect on non-small cell lung cancer and docetaxel had stronger inhibitory effect. Conclusion Blocking the Wnt signaling pathway can inhibit tumor growth. Docetaxel and Wnt-1 antibody have a positive effect on the inhibition of non-small cell lung cancer.