从二棱大麦 ( H ordeum distichon Linn.)幼苗中提取总 RNA,通过 RT- PCR获得目的片段 HDCS1 ,并采用 T-载体进行克隆。测序结果表明 ,HDCS1全长 664bp,含有 1个完整的阅读框架。其编码蛋白质全长 2 0 2个氨基酸 ,含有 8个由 1 1个氨基酸残基组成的基元序列 ,属第 3组 LEA蛋白。HDCS1与大麦 ( H ordeum vulgare L.) L EA蛋白、HVA1具有高度同源性 ,其差异在于 HDCS1比 HVA1缺少一个完整的由 1 1个氨基酸残基组成的基元序列 Total RNA was extracted from seedlings of H ordeum distichon Linn. The target fragment HDCS1 was obtained by RT-PCR and cloned using T-vector. Sequencing results show that, HDCS1 full-length 664bp, contains a complete reading frame. It encodes a protein of 220 amino acids in length and contains 8 motifs consisting of 11 amino acid residues and belongs to the third group LEA protein. HDCS1 is highly homologous to L EA protein and HVA1 of barley (H ordeum vulgare L.), with the difference that HDCS1 lacks a complete motif consisting of 11 amino acid residues compared to HVA1