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目的:建立用高效液相色谱法测定抗菌消炎胶囊中绿原酸含量的方法。方法:Betasil C18柱,流动相为乙腈-0．4％磷酸溶液(13:87),流速1．0 ml·min-1,检测波长为327 nm。结果:绿原酸在0．12～0．61 μg之间呈良好的线性关系(r= 0．999 7)。平均回收率100．5％(RSD=0．9％,n=5)。结论:所建立的方法稳定可靠灵敏、准确、简便,可用于抗菌消炎胶囊的质量控制。
Objective: To establish a method for determination of chlorogenic acid in antibacterial and anti-inflammatory capsules by high performance liquid chromatography. Method: Betasil C18 column, mobile phase of acetonitrile-0.4% phosphoric acid solution (13:87), flow rate 1.0 ml·min-1, detection wavelength of 327 nm. Results: The chlorogenic acid showed a good linear relationship between 0.12 and 0.61 μg (r= 0.999 7). The average recovery rate was 100.5% (RSD=0.9%, n=5). Conclusion: The established method is stable, reliable, accurate, simple, and can be used for quality control of antibacterial anti-inflammatory capsules.