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白细胞介素－１１（Ｉｎｔｅｒｌｅｕｋｉｎ－１１，ＩＬ－１１）是一种新的造血生长因子，体内、外实验结果显示ＩＬ－１１具有显著的造血促进作用，它能作用于早期的造血干细胞及祖细胞，促进其增殖和分化。为了探讨ＩＬ－１１基因治疗放射损伤后造血恢复的促进作用，我们构建了含人ＩＬ－１１ｃＤＮＡ的逆转录病毒载体，并转染小鼠成纤维细胞系ＮＩＨ－３Ｔ３，经筛选获得３株高表达ＩＬ－１１的转染细胞亚克隆，用高表达ＩＬ－１１的转染细胞作为载体细胞，进行了ＩＬ－１１基因治疗对放射损伤小鼠造血系统影响的实验研究。结果表明ＩＬ－１１基因治疗能明显缩短亚致死量放射损伤小鼠白细胞降低的时间，促进外周血白细胞、血小板和红细胞压积的恢复。提示ＩＬ－１１基因治疗对放射损伤造成的造血功能低下具有潜在的应用价值。
Interleukin-11 (IL-11) is a new hematopoietic growth factor. In vitro and in vivo experiments showed that IL-11 has a significant hematopoietic promoting effect and can act on early hematopoietic stem and progenitor cells , Promote their proliferation and differentiation. To investigate the role of IL-11 gene in promoting hematopoietic recovery after radiation injury, we constructed retroviral vector containing human IL-11 cDNA and transfected it into mouse fibroblast cell line NIH-3T3. Three highly expressed IL-11 transfected cells were subcloned, and the transfected cells with high expression of IL-11 were used as the carrier cells to study the effects of IL-11 gene therapy on the hematopoietic system in irradiated mice. The results showed that IL-11 gene therapy can significantly reduce the time of leukopenia in the sublethal radiation-injured mice and promote the recovery of peripheral white blood cells, platelets and hematocrit. These results suggest that IL-11 gene therapy has potential value in hematopoietic function caused by radiation injury.