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为研究富马酸二甲酯对斑马鱼(Danio rerio)胚胎早期发育的影响,选取不同发育阶段的斑马鱼胚胎,用富马酸二甲酯进行染毒处理,观察胚胎形态发育的异常,计算其对不同发育时期胚胎的24 h、48 h半数致死浓度(LC50)和胚胎72 h孵化率,并考察富马酸二甲酯对胚胎血管发育的影响。结果表明,富马酸二甲酯影响斑马鱼胚胎的早期发育,呈剂量依赖性特点,并与开始处理的时间点有关。富马酸二甲酯引起2 hpf(受精后2 h,2 hours post-fertilization)、10 hpf、24 hpf斑马鱼胚胎死亡的24 h LC50值分别为:13.33μmol/L、17.98μmol/L、32.50μmol/L,48 h LC50值分别为:13.31μmol/L、16.35μmol/L、22.50μmol/L;长期低浓度富马酸二甲酯(≥6μmol/L)作用引起胚胎72 h孵化率下降。27.5μmol/L富马酸二甲酯作用后会显著降低胚胎血管内皮生长因子受体2(VEGFR2)的表达水平。
In order to study the effect of dimethyl fumarate on the early embryo development of Danio rerio, zebrafish embryos of different developmental stages were selected and treated with dimethyl fumarate to observe the morphological abnormalities of embryos. The results showed that the LC50 and 48 h incubation rates of embryos at different developmental stages were 24 h, 48 h and 72 h respectively. The effects of dimethyl fumarate on embryonic vascularization were also investigated. The results showed that dimethyl fumarate affected the early development of zebrafish embryos in a dose-dependent manner, which was related to the time point of starting treatment. The 24 h LC50 values of dimethyl fumarate induced embryo death at 2 hpf (2 h post-fertilization, 2 h post-fertilization), 10 hpf, 24 hpf were 13.33μmol / L, 17.98μmol / L, 32.50 The 48 h LC50 values were 13.31μmol / L, 16.35μmol / L and 22.50μmol / L, respectively. The effect of long-term low concentration dimethyl fumarate (≥6μmol / L) 27.5μmol / L dimethyl fumarate significantly decreased the expression of vascular endothelial growth factor receptor 2 (VEGFR2).