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目的 探讨大肠癌组织中胃泌素与癌基因c myc、c fos、rasp2 1表达和核仁组成区嗜银染色 (Ag NORs)计数的关系及其意义。方法 对 48例大肠癌病人癌组织进行检测 ,采用免疫组化S P法测胃泌素、c myc、c fos、和rasp2 1表达 ,改良银染法测AgNORs计数 ,并与癌旁 3cm、6cm粘膜及正常大肠粘膜作对照。结果 癌组织胃泌素、c myc、c fos、rasp2 1表达的阳性率和AgNORs计数均显著高于癌旁 3cm、6cm粘膜和正常大肠粘膜 (P <0 .0 1) ,高分化腺癌组胃泌素表达阳性率明显高于低分化腺癌和粘液腺癌组 (P <0 .0 5 ) ,大肠癌组织胃泌素阳性组中c myc、c fos的阳性率及AgNORs计数均明显高于胃泌素阴性组 (P <0 .0 5 ,P <0 .0 1,P <0 .0 1)。结论 部分大肠癌细胞可自分泌产生胃泌素 ,介导调节c myc、c fos和rasp2 1癌基因扩增 ,从而促进癌细胞的增殖
Objective To investigate the relationship between gastrin and the expression of oncogenes c myc, c fos, and rasp21 in colorectal cancer and the arion tone count (Ag NORs) in karyotype. Methods Forty-eight colorectal cancer patients were detected. Immunohistochemical SP method was used to measure the expression of gastrin, c myc, c fos, and rasp2 1. The silver staining method was used to measure the AgNORs counts. The tumors were adjacent to the adjacent 3 cm and 6 cm mucosa. And normal colon mucosa as a control. Results The positive rates of gastrin, c myc, c fos, and rasp2 1 expression and AgNORs counts in the cancer tissues were significantly higher than those in the adjacent cancerous 3 cm and 6 cm mucosa and normal colorectal mucosa (P < 0.01). The positive rate of gastrin expression was significantly higher than that of poorly differentiated adenocarcinoma and mucinous adenocarcinoma (P < 0.05). The positive rates of c myc and c fos and the counts of AgNORs in the gastrin-positive group of colorectal cancer were significantly higher. In the gastrin-negative group (P <0.05, P <0.01, P <0.01). Conclusion Some colorectal cancer cells can produce gastrin autocrinely and regulate the proliferation of c myc, c fos and rasp2 1 oncogenes, thus promoting the proliferation of cancer cells.