本文研究了长期喂饲杀真菌剂代森锰、代森锌对小鼠精细胞内自由钙离子浓度[Ca~(2+)]:的影响。取6周雄性B 6 D 2 F 1小鼠(每组6只),分别在饲料中加入浓度为30,300,3000mg/kg的杀真菌荆代森锰:代森锌=1:1(w/w),喂养15周后取精子测定[Ca~(2+)]i。收集小鼠附皋精子于离心管中,1000 g离心7 min,弃去上清液,此时精子活动度为89±4％。将精子转入含2％小牛血清蛋白的Brinster氏介质中,混匀,离心并弃去上清液,调整浓度为每ml1.2×10~7个细胞左右(pH 7.65)。将精细胞在37℃用荧光染料Fura-2甲酯标记60 min(最后浓度为2.0 μmol/L),此时精子活动度为50±9％。再加入EGTA(3.3 mmol/L)和 In this paper, the effect of long-term feeding fungicide dexmedetomidine and dexamethasone on the intracellular free Ca2 + concentration [Ca2 +] in mice was studied. Six 6-week-old male B 6 D 2 F 1 mice (6 mice in each group) were taken and the fungicide Methansenoside at 30, 300, and 3000 mg / Sperm taken after 15 weeks of feeding [Ca ~ (2 +)] i. Mouse spermatozoa were collected in a centrifuge tube and centrifuged at 1000 g for 7 min. The supernatant was discarded and the sperm motility was 89 ± 4%. Sperm into Brinster medium containing 2% bovine serum albumin, mix, centrifuge and discard the supernatant to adjust the concentration of 1.2 × 10 ~ 7 cells per ml (pH 7.65). Sperm cells were labeled with the fluorescent dye Fura-2 methyl ester at 37 ° C for 60 min (final concentration 2.0 μmol / L) at which time sperm activity was 50 ± 9%. Then add EGTA (3.3 mmol / L) and