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目的 :研究化疗药物氟尿嘧啶 (5 - FU)、丝裂霉素 (MMC)、阿霉素 (Dox)、顺铂 (Cis)对人胃癌细胞株 (SGC)的作用特征 ,探讨 SGC细胞株对单一及联合化疗药物的敏感性 ,化疗药物浓度及作用时间对细胞抑制率的影响。方法 :SGC细胞株在含有不同药物的不同浓度的培养基中孵育不同时间 ,用 MTT法测定细胞的抑制率 ,化疗药物浓度参照其在人体的最高峰值浓度 ,选择其峰值浓度的 1/2 5～ 2倍范围 ,作用时间选择 2～ 2 4h。结果 :SGC细胞株对不同化疗药物的敏感性存在差异 ,对Dox最敏感 ,其次为 5 - FU、MMC,对 Cis不敏感。 5 - FU + MMC、5 - FU + Cis及 5 - FU + Dox+ MMC的联合应用表现了较明显的协同作用 ,其中 5 - FU、Dox及 MMC三药联用最显著 ;尽管 Cis单用不敏感 ,在联合应用中也表现了较强的协同作用。相同药物作用时间下 ,随着药物浓度的增加 ,Dox、5 - FU和 MMC对 SGC细胞株的抑制率明显增加 ,Cis的抑制率无明显变化 ;相同浓度下 ,药物作用时间对 SGC细胞株的抑制率也有显著影响 ;化疗药物的联合应用也表现了相似的特征。结论 :SGC细胞株对不同化疗药物的敏感性不同 ,化疗药物的联合应用可明显提高对细胞株的抑制率 ,单用不敏感的药物 ,联合应用中也可能表现较明显的协同作用 ;在影响药效动力学的主要?
Objective: To study the effect of chemotherapeutic agents such as 5 - FU, MMC, Dox and Cis on human gastric cancer cell line SGC, And the sensitivity of combined chemotherapy drugs, the concentration of chemotherapy drugs and the effect of time on the cell inhibition rate. Methods: SGC cell lines were incubated with different concentrations of different drugs in different culture medium for different time. MTT assay was used to determine the cell inhibitory rate. The concentration of chemotherapeutic drugs was calculated according to the highest peak concentration in human body. ~ 2 times the scope of the role of choice 2 ~ 2 4h. Results: The sensitivity of SGC cell lines to different chemotherapy drugs was the highest, most sensitive to Dox, followed by 5 - FU and MMC, but not to Cis. The combination of 5 - FU + MMC, 5 - FU + Cis and 5 - FU + Dox + MMC showed the most obvious synergistic effect. The combination of 5 - FU, Dox and MMC was the most significant. , In the joint application also showed a strong synergy. Under the same drug action time, with the increase of drug concentration, the inhibitory rate of Dox, 5 - FU and MMC on SGC cell line increased obviously, and the inhibition rate of Cis had no obvious change. Under the same concentration, the effect of drug on SGC cell line Inhibition rate also had a significant impact; the combination of chemotherapy drugs also showed similar characteristics. Conclusion: The sensitivity of SGC cell lines to different chemotherapeutic drugs is different. The combination of chemotherapeutic drugs can significantly increase the inhibition rate of cell lines, and the insensitive drug alone can exert synergistic effects in the combination therapy. Pharmacodynamics of the main?