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应用传代培养的牛和人主动脉内皮细胞及原代培养的人脐静脉内皮细胞为实验模型,用1%纤维蛋白的琼脂薄膜复盖于融合成单层的细胞上,观察比较蒲黄、丹参对纤维蛋白损伤牛和人血管内皮细胞的影响。发现牛和人主动脉或脐静脉内皮细胞接触含纤维蛋白的琼脂薄膜后,部分细胞出现收缩移动和细胞间隙增宽,随后大多细胞脱落,少数聚集成团;而加入蒲黄血清或丹参针液的防治组,大部分细胞形态基本正常。加入蒲黄、丹参防治组的培养液中,LDH及ACP含量均明显低于实验对照组。提示蒲黄、丹参均能减轻纤维蛋白凝块对血管内皮的损伤作用。
The cultured bovine and human aortic endothelial cells and primary cultured human umbilical vein endothelial cells were used as experimental models, and were overlayed with 1% fibrin agar films on cells fused into monolayers to observe the comparison of Puhuang and Salvia miltiorrhiza. Effects on fibrin-injured bovine and human vascular endothelial cells. After exposure to bovine and human aortic or umbilical vein endothelial cells in contact with fibrin-containing agar films, some cells showed contractile movement and widening of the intercellular spaces, followed by the loss of most of the cells, and a few aggregated into clusters; the addition of Puhuang serum or Salvia miltiorrhiza solution In the prevention and treatment group, most of the cell types were normal. The contents of LDH and ACP were significantly lower in the culture fluids of the control groups of Puhuang and Salvia miltiorrhiza than in the experimental control group. It is suggested that Puhuang and Salvia miltiorrhiza can all reduce the damage of fibrin clot to vascular endothelium.