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本研究目的在于对96份海南高良姜种质进行遗传多样性和亲缘关系分析,采用了ISSR分子标记技术对其进行扩增,并利用NTSYS-pc 2.10 y等软件进行数据统计。共利用22条引物扩增获得了154条条带,其中有97条多态性条带,引物多态性为61.88%。96分高良姜种质材料的DICE相似系数在0.808~0.996之间,平均值为0.931。基于UPGMA算法基础上构建的聚类进化树显示,大部分的材料在相似系数阈值接近0.87时聚成两大类,其中一大类几乎包括了来自海南的所有材料,主成分分析同时验证了聚类分析的结果。以上研究说明海南高良姜栽培种的遗传多样性较低,此研究可为高良姜种质资源的收集、分类、以及GAP基地建设等提供一定的理论依据。
The aim of this study was to analyze the genetic diversity and genetic relationship of 96 Alpinia officinarum Hainan germplasms. The ISSR molecular marker technique was used to amplify the genetic diversity and NTSYS-pc 2.10 y software was used for data statistics. A total of 154 bands were amplified with 22 primers, of which 97 were polymorphic bands with a primer diversity of 61.88%. The DICE similarity coefficient of 96 points Alpinia officinarum germplasm ranged from 0.808 to 0.996 with an average of 0.931. The clustering phylogenetic tree based on the UPGMA algorithm shows that most of the materials are clustered into two groups when the similarity coefficient threshold is close to 0.87. One of the major categories includes almost all the materials from Hainan. The principal component analysis also verifies that poly The result of class analysis. The above study shows that the genetic diversity of cultivars of Galangal is low in Hainan. This study may provide some theoretical basis for the collection, classification of Gallnut germplasm resources and the construction of GAP bases.