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目的比较汽油汽车燃烧尾气和甲醇汽车燃烧尾气对肺巨噬细胞免疫功能的影响,为筛选清洁能源提供依据。方法利用肺巨噬细胞受体能与致敏的绵羊红细胞反应形成玫瑰花环并有一定的结合率,将灌洗出的兔肺巨噬细胞培养一定时间后再按0.500、0.250、0.125、0.062、0.031 L/ml浓度将汽油和甲醇燃烧尾气的提取物对巨噬细胞染毒,在37℃、5%CO2孵箱中培养3 h后加入致敏的绵羊红细胞,放入冰箱内反应2 h。取出后计数玫瑰花环形成率。并进行了抗体介导的细胞毒作用试验。结果汽油和甲醇燃烧尾气作用都使巨噬细胞花环形成率降低,未染毒组的结合率为85.7%,汽油和甲醇在最低试验浓度0.031 L/ml时花环结合率分别为47.0%和70.0%,汽油燃烧尾气结合率明显低于甲醇燃烧尾气;汽油尾气提取物对抗体介导的细胞毒作用有显著的抑制作用,汽油燃烧尾气使其降低的幅度明显大于甲醇燃烧尾气。结论甲醇燃烧尾气对巨噬细胞的免疫功能的影响明显小于汽油燃烧尾气,所以更适合做清洁能源。
Objective To compare the effects of gasoline tail gas and methanol tail gas on immune function of lung macrophages in order to provide basis for screening clean energy. Methods Receptor of pulmonary macrophages can react with sensitized sheep erythrocytes to form rosette and have a certain binding rate. After lavage of rabbit pulmonary macrophages for a certain period of time, the macrophages were incubated for 0.500, 0.250, 0.125 and 0.062, 0.031 L / ml concentration of gasoline and methanol combustion exhaust extract on macrophages, cultured in 37 ℃, 5% CO2 incubator for 3 h after adding sensitized sheep erythrocytes and placed in the refrigerator for 2 h. After removing rose ring formation count rate. Antibody-mediated cytotoxicity assays were performed. Results The rate of formation of rosette of macrophages was decreased by gasoline and methanol tail gas. The rate of rosette formation was 85.7% in non-exposed group and 47.0% and 70.0% in gasoline and methanol at the lowest concentration of 0.031 L / ml, respectively , The binding rate of gasoline combustion exhaust gas was significantly lower than that of methanol combustion exhaust gas extract; gasoline exhaust extract significantly inhibited the antibody-mediated cytotoxicity, gasoline combustion exhaust gas to significantly reduce the extent of the combustion of methanol exhaust. Conclusion Methanol combustion tail gas on immune function of macrophages was significantly less than gasoline combustion exhaust, so more suitable for clean energy.