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玉米的紫胚标记是一种有效的鉴定单倍体与加倍单倍体的胚标记手段。如果能够从分子水平寻找到与紫胚性状相关联的标记,则能更加快捷,简便,准确地对单倍体进行鉴定。在本研究中,我们从包含6种单倍体与3种加倍单倍体的玉米样品中提取基因组DNA,并对其进行PCR扩增以及电泳,随后对9种样品进行遗传相似性分析,得出其亲缘关系图谱。和其他基于PCR技术的DNA指纹技术相比,RAPD具有方便,快捷和成本低的优点,因此,在本实验中我们选择了RAPD来进行研究。
The purple embryo marker of maize is an effective means of embryo marker identification of haploid and doubled haploid. Haplotypes can be identified more quickly, easily and accurately if they are able to find the markers associated with purple embryo traits at the molecular level. In this study, genomic DNA was extracted from maize samples containing 6 haploids and 3 doubling haploids, and PCR amplified and electrophoresed, followed by genetic similarity analysis of 9 samples Out of their kinship map. Compared with other DNA fingerprinting based on PCR technology, RAPD has the advantages of convenience, quickness and low cost. Therefore, we chose RAPD in this experiment.