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构建编码ＨＢｓＡｇ 蛋白的重组真核表达质粒ｐＣＲ３－１Ｓ作为ＨＢＶ 基因疫苗， 免疫接种Ｂａ１ｂ／ｃ 小鼠。以重组质粒ｐＣＲ３－１Ｓ转染的Ｓｐ２／０ 细胞作为靶细胞， 采用５１Ｃｒ ４ｈ 释放法， 体外检测免疫小鼠的淋巴细胞杀伤功能。结果显示， 与空载体对照组相比较， ＨＢＶ基因疫苗可诱导Ｂａｌｂ／ｃ 小鼠产生ＨＢＶ 特异性细胞毒性Ｔ 细胞应答（ Ｐ＞ ０－０５） ， 提示以Ｓｐ２／０ 基因转染的细胞作为靶细胞， 检测免疫Ｂａｌｂ／ｃ 小鼠淋巴细胞的杀伤功能是可靠的。
Construction of recombinant eukaryotic expression plasmid pCR3-1S encoding HBsAg protein as HBV gene vaccine, immunization Ba1b / c mice. Sp2 / 0 cells transfected with the recombinant plasmid pCR3-1S were used as target cells. The 51Cr 4h release method was used to detect the cytotoxicity of lymphocytes in immunized mice. The results showed that HBV gene vaccine could induce HBV-specific cytotoxic T cell response in Balb / c mice compared with the control group (P> 0-05), suggesting that cells transfected with Sp2 / 0 gene could be used as targets Cell, Detecting Immune Balb / c mouse lymphocyte killing function is reliable.