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对何首乌的组培快繁技术进行了研究。结果表明 :采用 MS+6-BA1 .0 mg/L+IBA0 .1 mg/L的培养基能成功地诱导芽的分化。对于芽增殖 ,以 MS+6-BA 0 . 75 mg/L+IBA 0 .0 5 mg/L或MS+6-BA 1 .5 mg/L+IBA 0 .1 mg/L培养基较好 ,培养 3 0 d后芽增殖率可分别达到 3 .89和 4.1 1。诱导生根以 1 /2 MS+IBA 0 .5～ 1 .2 5 mg/L或 1 /2 MS+NAA 0 .5 mg/L培养基较好 ,培养 2 0 d后生根率可达80 .0 %～ 86.7%。
Polygonum multiflorum Tissue culture and propagation techniques were studied. The results showed that bud differentiation could be successfully induced by MS + 6-BA1.0 mg / L + IBA0.1 mg / L medium. For shoot multiplication, it is better to use MS + 6-BA 0.75 mg / L + IBA 0 .05 mg / L or MS + 6-BA 1.5 mg / L + IBA 0.1 mg / After 30 days of culture, the shoot proliferation rate could reach 3.89 and 4.1 1 respectively. The induction of rooting with 1/2 MS + IBA 0.5 ~ 1.25 mg / L or 1/2 MS + NAA 0.5 mg / L medium was better, the rooting rate reached 80.0 after culture for 20 days % ~ 86.7%.