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目的 观察血小板活化因子受体拮抗剂WEB2 0 86对载脂蛋白E基因敲除鼠斑块内血管新生和斑块面积的影响。方法 8周龄雄性载脂蛋白E基因敲除鼠普通饲料喂养2 4周,然后随机分为对照组和WEB2 0 86组2组,每组18只。两组均普通饲料,对照组普通饮水,WEB2 0 86组则在普通饮水中加入血小板活化因子受体拮抗剂WEB2 0 86 (43mg/L)。继续喂养8周后,空腹18h采血,安乐死,取材。全自动生物化学分析仪检测血清脂质含量,CD31全层铺片检测斑块内毛细血管密度,含斑块的主动脉环置于matrigel中培养检测斑块新生毛细血管的能力,苏丹Ⅳ染色检测斑块面积。结果 WEB2 0 86对血清脂质无明显影响。WEB2 0 86明显抑制粥样斑块内毛细血管平均密度(34.6 %±10 .2 %比16 .1%±6 .7% ,P <0 .0 1)、明显抑制含斑块的主动脉环在matrigel中新生毛细血管(172 .3±4 0 .6比73.1±2 4 .9,P <0 .0 5 )、显著减小主动脉斑块面积(31.4 %±9.7%比17.5 %±6 .3% ,P <0 .0 1)。结论 WEB2 0 86对血脂无明显影响,但可抑制斑块内血管新生和减小斑块面积。
Objective To investigate the effects of platelet-activating factor receptor antagonist WEB2 0 86 on angiogenesis and plaque area in apolipoprotein E knockout mice. Methods Eight-week-old male apolipoprotein E gene knockout mice were fed with normal diet for 24 weeks and then randomly divided into control group and WEB2 0 86 group with 18 rats in each group. Both groups were fed the normal diet, the control group drinking water, WEB2 0 86 group in ordinary drinking water added platelet-activating factor receptor antagonist WEB2 086 (43mg / L). Continue feeding for 8 weeks, fasting 18h blood, euthanasia, drawing. The serum lipid level was detected by automatic biochemical analyzer. The density of capillaries in the plaque was detected by CD31 full-thickness patch. The plaque-containing aortic rings were placed in matrigel to detect the ability of plaque neovascularization. Sudan Ⅳ staining Plaque area. Results WEB2 0 86 had no significant effect on serum lipids. WEB2 0 86 significantly inhibited the average density of capillaries in atherosclerotic plaque (34.6% ± 10.2% vs 16.1% ± 6.7%, P <0.01), and significantly inhibited plaque-containing aortic rings Renal capillaries in matrigel (172.3 ± 40.6 vs 73.1 ± 24.9, P <0.05) significantly reduced aortic plaque area (31.4% ± 9.7% vs. 17.5% ± 6 .3%, P <0. 01). Conclusion WEB2 0 86 has no significant effect on blood lipids but inhibits angiogenesis and plaque area in plaque.