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在胆碱能受体拮抗剂东莨菪碱具有抗抑郁活性的临床报道启发下,本研究评价了东莨菪碱在小鼠模型上的抗抑郁活性及其有效剂量,并观察其在抗抑郁有效剂量下对中枢神经系统和学习记忆功能的影响。与溶剂对照组相比,东莨菪碱在0.1～0.4 mg·kg-1剂量范围内能够显著缩短小鼠悬尾实验中的不动时间(P<0.01或P<0.001),并显著缩短小鼠强迫游泳实验中的不动时间(P<0.001),但在跳台实验中对小鼠跳台潜伏期和错误次数无明显影响;0.1和0.2 mg·kg-1剂量下在开阔场实验中对小鼠自主活动无明显影响,0.4 mg·kg-1剂量下显著增加小鼠自主活动。结果提示,东莨菪碱对小鼠具有良好的抗抑郁活性,0.1和0.2 mg·kg-1是其抗抑郁作用的最佳有效剂量,在此剂量下对小鼠的学习记忆能力无明显影响,对中枢神经系统亦无明显兴奋或抑制作用。
Inspired by clinical reports that cholinergic receptor antagonist scopolamine has antidepressant activity, this study evaluated the antidepressant activity and effective dose of scopolamine in a mouse model and evaluated the effect of scopolamine on the central nervous system System and learning and memory functions. Compared with the solvent control group, scopolamine significantly shortened the immobility time (P <0.01 or P <0.001) in mouse tail suspension test and significantly shortened forced swimming of mice in the dose range of 0.1-0.4 mg · kg-1 (P <0.001). However, there was no significant effect on jumping latency and number of mistakes in jumping platform test. No significant difference was observed in the spontaneous activity of mice in the open field at 0.1 and 0.2 mg · kg-1 doses Significant effect, 0.4 mg · kg-1 dose significantly increased autonomic activity in mice. The results suggest that scopolamine has good antidepressant activity in mice, 0.1 and 0.2 mg · kg-1 is the best effective dose of its antidepressant effect, at this dose had no significant effect on learning and memory in mice, the central No obvious nervous system nervous or inhibitory effect.