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本文对间接血凝试验诊断日本血吸虫病的实验方法和影响因素进行了研究.实验证明:不论采用1％或2.5％鞣化红细胞,加入1％正常兔血清(Normal rabbit serum,简称1％NRS)后,可以消除鞣化红细胞的自凝现象.比较鞣化红细胞加入1％NRS后再致敏与致敏红细胞加入1％NRS后对间接血凝试验的影响,结果表明:二者对间接血凝试验的敏感性无显著差异(P>0.05).不同致敏红细胞浓度对间接血凝试验的影响,实验结果显示:采用1％致敏红细胞试液可提高间接血凝试验的敏感性;采用2.5％浓度则可提高间接血凝试验的特异性.故在血吸虫病重流行区,为了提高间接血凝试验的特异性,可考虑用2.5％浓度的致敏红细胞试液,而在非流行区及轻流行区,为了提高间接血凝试验的敏感性,可采用1％浓度,以节省致敏红细胞的用量.温度与间接血凝试验反应速度的关系是:随着温度的升高,间接血凝试验反应速度加快,观察结果的时间可以相应缩短.
In this paper, experimental methods and influencing factors for the diagnosis of schistosomiasis japonica by indirect hemagglutination test were studied. Experiments have shown that 1% normal rabbit serum (1% NRS) is added regardless of whether 1% or 2.5% of erythrocytes are used. After that, the self-condensation of the red blood cells can be eliminated. The effect of sensitization and sensitization of sensitized red blood cells with 1% NRS on the indirect hemagglutination test after addition of 1% NRS to selenium red blood cells is shown. The sensitivity of the test was not significantly different (P>0.05). The effect of different concentrations of sensitized red blood cells on the indirect hemagglutination test showed that the sensitivity of the indirect hemagglutination test could be improved with a 1% sensitized red blood cell test; 2.5 was used. % concentration can increase the specificity of the indirect hemagglutination test. Therefore, in order to increase the specificity of the indirect hemagglutination test in the epidemic area of schistosomiasis, consider using a 2.5% concentration of sensitized red blood cell test solution in non-endemic areas. In light endemic areas, in order to increase the sensitivity of the indirect hemagglutination test, 1% concentration may be used to save the amount of sensitized red blood cells. The relationship between temperature and indirect hemagglutination test reaction rate is: as the temperature increases, indirect hemagglutination test The reaction speed is quickened, and the observation time can be shortened accordingly.