Daisy Miller, among Henry James’masterpieces, is the novella which won him international fame. As some critics have analyzed, Daisy is a victim of the values of the Old World. In the author’s opinion, Daisy’s tragedy should be partly attrib-uted to prejudice, which impedes the international communication between the newly wealthy Americans and the Europeanized Americans. In this sense, this paper focuses on an analysis of three forms of prejudice in Daisy Miller and tries to interpret Daisy’s tragic destiny from the perspective of intercultural communication.