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目的：探讨一种测定人与小鼠自然杀伤细胞（ＮＫ）杀伤活性的方法。方法：采用ＮＡＧ微量酶比色法，对此法应用的最适条件进行了系统的研究，对正常人不同年龄组进行数次实验，对Ｂａｌｂ／ｃ小鼠也进行了不同浓度的测定实验。结果：正常人的效应细胞与靶细胞（Ｋ５６２）比例为１０∶１时，效应细胞浓度为２×１０６，孵育时间为２０ｈ，以测定效应细胞和靶细胞培养上清中Ｎ乙酰βＤ氨基己糖酶活性，作为ＮＫ杀伤活性的指标，是测定系统的最适条件。小鼠（Ｂａｌｂ／ｃ）效靶比例与人的相似，最适比例为１０∶１或２０∶１。结论：本法有一定的实用价值，无同位素危害，具有较好的敏感性、稳定性、可重复性。
Objective: To investigate a method for determining the killing activity of natural killer (NK) cells in mice and humans. Methods: NAG micro-enzyme colorimetric method was used to study the optimal conditions for this method. Several experiments were conducted in different age groups of normal people and different concentrations of Balb / c mice were also tested. Results: When the ratio of effector cells to target cells (K562) was 10: 1, the effector cell concentration was 2 × 106 and the incubation time was 20h. To determine the effect of Nacetylββ in effector cells and supernatant of target cells D-amino hexosaminidase activity, as an indicator of NK cytotoxic activity, is the optimum condition for the assay system. Mouse (Balb / c) target ratio similar to human, the most appropriate ratio of 10: 1 or 20: 1. Conclusion: This method has some practical value, no isotope hazards, with good sensitivity, stability and repeatability.