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目的采用超高效液相色谱法(UPLC)同时测定益智药材中的白杨素和圆柚酮。方法色谱柱为Acquity UPLC BEH C18柱(100 mm×2.1 mm,1.7μm),流动相为乙腈-水溶液,流速0.2 m L·min-1,采用梯度洗脱,检测波长251 nm。结果白杨素0.139~347.5μg·m L-1、圆柚酮0.197~492.5μg·m L-1与峰面积呈良好的线性关系(r2分别为0.9995、0.9994)。平均加样回收率分别为98.97%(RSD=1.94%,n=9)、98.90%(RSD=1.45%,n=9)。不同地区的益智和益智的不同部位中两种成分的含量均有明显差异。结论所用方法准确、简单、重复性好,适用于益智中白杨素和圆柚酮的含量测定。
OBJECTIVE To simultaneous determination of chrysin and chrysin in kadsura by ultra performance liquid chromatography (UPLC). Methods The column was Acquity UPLC BEH C18 (100 mm × 2.1 mm, 1.7 μm). The mobile phase consisted of acetonitrile-water and the flow rate was 0.2 m L · min-1. The gradient elution was performed with a detection wavelength of 251 nm. Results Choline was 0.139 ~ 347.5μg · m L-1, and 0.197 ~ 492.5μg · m L-1 of pomeloone had a good linear relationship with the peak area (r2 = 0.9995,0.9994, respectively). The average recoveries were 98.97% (RSD = 1.94%, n = 9) and 98.90% (RSD = 1.45%, n = 9), respectively. There are significant differences in the content of the two components in different parts of the puzzle and puzzle in different regions. Conclusion The method is accurate, simple and reproducible. It is suitable for the determination of chrysin and pombene in puzzle.