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目的 :研制乙型肝炎肝纤维化的动物模型。方法 :采用 1d龄樱桃谷鸭 ,随机分为正常组、鸭乙型肝炎病毒 (DHBV )造模组及 CCl4造模组 ,分别于 6 0、10 0及 112 d随机抽样观察两造模组鸭肝组织病理变化 ,于 10 0 d检测 3组动物肝组织 DHBV、DNA、肝功能和肝纤维化指标。结果 :CCl4造模组脂肪变性明显 ,肝细胞水样变性伴中等炎性细胞浸润 ,纤维组织增生明显 ;DHBV造模组以水样变性为主 ,可见肝窦消失或肝窦扩张瘀血 ,脂肪变性以小脂滴为主 ,肝细胞坏死程度较轻 ,但炎性细胞浸润明显 ,且以淋巴细胞多见 ,纤维增生形成时间略长 ,6 0 d尚未见纤维增生 ,80 d时汇管区纤维组织中度增生 ,10 0 d和 112 d时纤维组织明显增生 ,有的形成假小叶 ,其纤维化形成率与 CCl4组 (90 % )接近 (10 0 d为 6 0 .7% ,112 d为 87.5 % )。肝组织 DHBV DNA检测结果 ,DHBV造模组水平较高 ,其肝功能 AL T正常 ,白蛋白明显降低 ,球蛋白显著升高 ,肝纤维化指标如 HA、PC 和肝组织 Hyp均明显上升。结论 :采用 DHBV阳性血清反复攻击樱桃谷鸭 112 d以上 ,可复制形成率较高的鸭乙型肝炎肝纤维化模型
Objective: To develop an animal model of hepatitis B liver fibrosis. Methods: The 1-day-old Cherry Valley duck was randomly divided into normal group, DHBV model group and CCl4 model group. The ducklings were randomly selected at 60, 100 and 112 days respectively The pathological changes of liver tissue were examined. DHBV, DNA, liver function and liver fibrosis indexes of liver tissue were detected in the 10 days. Results: CCl4-induced fatty degeneration was obvious, the degeneration of hepatocytes was associated with moderate inflammatory cell infiltration and fibrous tissue hyperplasia. The degeneration of DHBV was mainly caused by watery degeneration, Denaturation was mainly composed of small lipid droplets, with mild necrosis of liver cells, but infiltration of inflammatory cells was obvious. More frequent lymphocytosis, slightly longer fibrogenesis, no fibrogenesis on 60 days, The tissue hyperplasia, fibrous tissue hyperplasia on the 100th and 112th days, and the formation of false lobules. The formation rate of fibrosis was close to that of CCl4 group (90%) (60.7%, 112 days, 87.5%). The results of DHBV DNA test showed that the DHBV model group had higher levels of ALT, normal ALT, lower albumin, higher globulin and higher hepatic fibrosis indexes such as HA, PC and liver Hyp. Conclusions: Duck hepatitis B liver fibrosis model can be replicated with DHBV-positive serum over 112 days