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通过盆栽试验,研究黄瓜组织浸提液对黄瓜幼苗生长、根际土壤微生物数量、酶活性的化感作用。结果表明,随着黄瓜植株浸提液浓度的增加,黄瓜幼苗的株高、子叶大小、地上部干重、根干重表现为先增加后降低,而丙二醛含量先降低后增加;黄瓜根际土壤中放线菌数量显著减少25.5%,真菌、镰刀菌数量先减少后分别增加1.55%、137%,细菌数量先增加51.9%后减少12.7%;多酚氧化酶、脲酶、过氧化氢酶活性显著增加,而蔗糖酶活性显著降低。相关分析表明,多酚氧化酶活性与脲酶、过氧化氢酶活性显著正相关,与蔗糖酶活性、真菌数量显著负相关;脲酶活性与过氧化氢酶活性、镰刀菌数量显著正相关,与放线菌数量显著负相关;蔗糖酶活性与真菌数量显著正相关,过氧化氢酶活性与放线菌数量显著负相关,与镰刀菌数量显著正相关。本试验说明黄瓜组织中的化感物质影响黄瓜幼苗的生长、改变土壤的生物化学结构。
Pot experiments were conducted to study the allelopathic effects of cucumber extracts on cucumber seedling growth, rhizosphere soil microbial quantity and enzyme activity. The results showed that the plant height, cotyledon size, shoot dry weight and root dry weight of cucumber seedlings increased first and then decreased with the increase of cucumber plant extract concentration, while the content of malondialdehyde first decreased and then increased; The number of actinomycetes in soil decreased significantly by 25.5%, while the number of fungi and Fusarium decreased first and then increased by 1.55% and 137%, respectively. The number of bacteria increased by 51.9% and then decreased by 12.7% respectively. Polyphenol oxidase, urease and catalase Significantly increased activity, while sucrase activity was significantly reduced. Correlation analysis showed that the activity of polyphenol oxidase was significantly and positively correlated with the activities of urease and catalase, but significantly negatively correlated with the activity of sucrase and the number of fungi. The activity of urease was significantly and positively correlated with the activity of catalase and the number of Fusarium, There was a significant negative correlation between the number of mycobacteria and invertase activity. The activity of invertase was positively correlated with the number of fungi. The activity of catalase was significantly negatively correlated with the number of actinomycetes and positively correlated with the number of Fusarium. This experiment shows that the allelochemicals in cucumber tissue affect the growth of cucumber seedlings and change the biochemical structure of soil.