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南美香瓜梨茎段被移植在MS基本培养基上,其中添加有BA6.0(mg/L以下单位相同)、IBA0.2培养4周后增殖到3.8倍。用其嫩叶切块,在含有2,4—D0.5,BA0.25的MS培养基上先诱导形成绿色愈伤组织,然后转入含BA6.0和IBA0.2的MS培养基上,产生丛生不定芽。将2.5~3.0cm的不定芽切下移入含IBA0.5与0.2%活性炭,1/2MS无机盐的培养基上,25天后诱导生根,移栽成活率达90%以上。
The shoots of South American melon pear were transplanted on MS basal medium with BA6.0 (same unit below mg / L), and IBA0.2 proliferated to 3.8 times after 4 weeks of culture. The green callus was first induced on its MS medium containing 2,4-D0.5 and BA0.25 by cutting with its young leaves, then transferred to MS medium containing BA6.0 and IBA0.2, Generate adventitious buds. The adventitious bud of 2.5-3.0cm was cut into medium containing IBA0.5 and 0.2% activated carbon and 1 / 2MS inorganic salt to induce rooting after 25 days, and the survival rate after transplanting was more than 90%.