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目的通过90 d喂养试验初步评价转人α-乳清白蛋白基因牛肉的食用安全性。方法将普通牛肉和转基因牛肉分别制成牛肉粉,按照5%的比例掺入基础饲料并调整配方使之与啮齿类实验动物纯化饲料AIN93G相一致。将Wistar大鼠按体质量随机分为3组,分别以转基因牛肉饲料、普通牛肉饲料和基础饲料AIN93G喂养90 d。每日观察动物体征,每周记录动物体质量和进食量,试验结束时对动物进行尿液检查、血液细胞学和血液生化学分析,对重要脏器称重并进行组织病理学检查。结果进食含有转基因牛肉的饲料对大鼠体质量、进食量、尿液、血液细胞学和血液生化学、脏器重量及组织病理学均未产生明显改变,差异均无统计学意义(P>0.05)。结论大鼠的90 d喂养试验结果提示转基因牛肉的食用安全性与普通牛肉无差别。
OBJECTIVE: To evaluate the food safety of human α-lactalbumin-transgenic beef by 90-day feeding experiment. Methods The normal beef and GM beef were respectively made into beef meal. The basic feed was mixed according to the ratio of 5% and the formula was adjusted to be consistent with that of rodent experimental animal purified feed AIN93G. The Wistar rats were randomly divided into three groups according to body weight, and fed with GM beef, common beef and basic diet AIN93G for 90 days respectively. Animals were observed daily for signs of body weight and food intake weekly. Urine tests, hematological and blood biochemical analyzes of animals were performed at the end of the trial, and vital organs were weighed and histopathologically examined. Results There was no significant difference in the body weight, food intake, urine, blood cytology, blood biochemistry, organ weight and histopathology of the rats fed with GM beef, the difference was not statistically significant (P> 0.05 ). Conclusion The result of 90-day feeding test in rats suggests that the safety of GM beef is not different from that of ordinary beef.