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抗病品种中棉12、86-1,感病品种岱15、豫棉1号在棉花幼苗三叶期用采自河南王屯的枯萎菌7号小种接种。在接种后48小时,抗病品种出现木质素前体阿魏酸的增加峰,最大可达4.27～6.68毫克/克干重,而感病品种在接菌后24～72小时,阿魏酸含量始终低于未接菌的对照,直到96小时才略有上升,最大位只有2.17～3.48毫克/克干重,表明阿魏酸含量的变化与棉花对枯萎病的抗性呈正相关。棉株体内酚类植保素—绿原酸的积累在抗、毒品种中表现亦不相同,在接种早期(24小时)抗病品种就出现了绿原酸增加峰,最大量为0.47～0.51毫克/克干重,感病品种在24～72小时绿原酸含量一直低于对照,至96小时才迅速增加,最大量为0.49～0.55毫克/克干重,结果表明,棉花品种抗感差异主要与这种酚类植保产生的量和速度有关。棉幼苗健、病株提取物可抑制枯萎菌生长,抗病健株提取物的抑菌效果优于感病健株,接菌后抗、感品种提取物抑菌作用减弱,并且抗、感之间差异减小。
The resistant varieties of cotton 12,86-1, susceptible varieties Dai 15, Yumian 1 in the three-leaf stage of cotton seedlings with Fusarium strain 7 from Henan Wangtun inoculation. At 48 hours after inoculation, the resistant peaks of ferulic acid lignin precursors increased up to 4.27 ~ 6.68 mg / g dry weight, while the susceptible cultivars 24 ~ 72 hours after inoculation, ferulic acid content Always lower than the non-inoculated control, until 96 hours before rising slightly, the maximum bit is only 2.17 to 3.48 mg / g dry weight, indicating that changes in ferulic acid content and resistance to Fusarium wilt was positively correlated. Cotton plant phenolic phytoalexin - chlorogenic acid accumulation in the anti-virus species did not show the same, early in the vaccination (24 hours) resistant varieties appeared chlorogenic acid increased peak, the maximum amount of 0.47 ~ 0.51 mg / G dry weight, susceptible varieties in 24 to 72 hours chlorogenic acid content has been lower than the control, only 96 hours to increase rapidly, the maximum amount of 0.49 ~ 0.55 mg / g dry weight, the results show that the main varieties of cotton resistance to differences It is related to the amount and speed of this phenolic plant protection. Cotton seedlings healthy and diseased plant extracts can inhibit the growth of Fusarium oxysporum, antibacterial effect of the disease-resistant strains better than susceptible strains, antibacterial effect of anti-susceptible, susceptible varieties extract after inoculation, and anti-sense Difference between the decrease.