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本文采用放射性配体结合方法观察大鼠心肌缺血与重灌时α_1肾上腺素受体及其亚型——α_(1a)与α_(1b)改变。结果显示左冠状动脉结扎后4小时,缺血心肌的α_1受体与~(125)I-BE结合的数量与亲和性不变,但由对照时含有α_(1a)与α_(1b)两种亚型转变为只含α_(1b)一种亚型。离体灌流心脏停灌30min后α_1受体及其亚型均无显著改变,当停灌30min后再重灌15min时虽然α_1受体的数量不变,但与~(125)I-BE结合的亲和性下降,同时也发生α_(1a)亚型向。α_(1b)亚型的转换。
In this paper, the changes of α 1 adrenergic receptor and its subtypes αα (1a) and α 1b (1b) were observed by radioactive ligand binding assay. The results showed that the number and affinity of α_1 receptor in ischemic myocardium did not change with the binding of ~ (125) I-BE at 4 hours after left coronary artery occlusion, but there was no change in the number and affinity of α_ (1a) and α_ (1b) The subtype is transformed into a subtype containing only alpha_ (1b). There was no significant change in α_1 receptor and its subtypes after 30 min perfusion in isolated hearts. When the number of α_1 receptors remained unchanged after 30 min reperfusion and 15 min reperfusion, the number of α 1 receptors bound to 125 I-BE Affinity decreased, but also occurred α_ (1a) subtype to. Conversion of α_ (1b) isoforms.