直接抗病毒药对慢性丙型肝炎患者外周血单个核细胞频率及其活化因子sCD14s、CD163的影响

来源 :中华肝脏病杂志 | 被引量 : 0次 | 上传用户:dianzishu1981
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目的:探索直接抗病毒药(DAAs)对慢性丙型肝炎患者外周血单个核细胞频率及其活化因子sCD14s、CD163的影响。方法:收集初治慢性丙型肝炎患者15例,健康对照者10名资料;慢性丙型肝炎患者给予DAAs治疗12周,分别在第0、4、12周收取血标本,健康对照者血标本作为对照。流式细胞术检测外周血经典型CD14n ++CD16n -单个核细胞和促炎型CD14n +CD16n +单个核细胞频率,用酶联免疫吸附法检测血清sCD14s、sCD163。计量资料两组间比较采用n t检验,多组间比较先行方差分析,进一步两两比较,用LSD-n t检验。n 结果:DAAs治疗前外周血CD14n +CD16n +单个核细胞频率(18.49%±1.54%比10.65%±0.83%)、血清sCD14s[ (64 407.38±5 778.49)pg/ml比(28 370.76±2 357.68)pg/ml]和sCD163[(22 853.80±4 137.61)pg/ml比(2 934.41±223.31)pg/ml)]均高于健康对照者(n P < 0.05),而外周血CD14 n ++CD16n -单个核细胞频率则低于健康对照者(59.14%±0.54%比72.75%±1.31%,n P 0.05),血清sCD14和sCD163仍高于健康对照者[sCD14:(44 390.06±3 330.17) pg/ml比(28 370.76±2 357.68) pg/ml, n P < 0.01;sCD163:(11 494.79±1 836.97)pg/ml比(2 934.41±223.31) pg/ml, n P 0.05)。n 结论:DAAs治疗可降低慢性丙型肝炎患者外周血单个核细胞的活化。“,”Objective:To explore the effects of direct antiviral agent (DAAs) on the frequency of peripheral blood mononuclear cells and their activating factors sCD14s and CD163 in patients with chronic hepatitis C.Methods:Data of 15 treatment-naive chronic hepatitis C patients and 10 healthy controls were collected. Patients with chronic hepatitis C were treated with DAAs for 12 weeks. Blood samples were collected at 0, 4 and 12 weeks respectively, and blood samples of healthy controls were used as controls. Flow cytometry was used to detect the frequency of classical CD14n ++CD16n - mononuclear cells and pro-inflammatory CD14n +CD16n + mononuclear cells in peripheral blood. Serum sCD14s and sCD163 were detected by enzyme-linked immunosorbent assay. The comparison between the two groups was performed by t-test. The comparison between multiple groups was performed by analysis of variance, and further pairwise comparison was performed by LSD-t test.n Results:Prior DAAs treatment, peripheral blood CD14n +CD16n + mononuclear cell frequency (18.49% ± 1.54% vs. 10.65% ± 0.83%), serum sCD14s [(64 407.38 ± 5778.49) pg/ml vs. (28 370.76 ± 2 357.68 ) pg/ml] and sCD163 [(22 853.80 ± 4 137.61) pg/ml vs. (2 934.41 ± 223.31) pg/ml] were all higher than healthy controls (n P < 0.05), while the frequency of CD14 n ++CD16n - mononuclear cells in peripheral blood was lower than healthy controls (59.14%±0.54% vs. 72.75%±1.31%, n P 0.05), and serum sCD14 and scd163 were still higher than those of healthy controls [sCD14: (44 390.06 ± 3 330.17) pg / ml vs. (28 370.76 ± 2 357.68) pg/ml, Scd163: (11 494.79 ± 1 836.97) pg / ml vs. (2 934.41 ± 223.31) pg / ml, n P 0.05).n Conclusion:DAAs therapy can reduce the activation of peripheral blood mononuclear cells in patients with chronic hepatitis C.
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