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目的:探讨阶段特异性胚胎抗原SSEA-3及阶段特异性胚胎抗原SSEA-4在乳腺良恶性肿瘤及乳腺癌细胞系MDA-MB-468中的表达情况,及其与乳腺癌发生的关系。方法:通过免疫组织化学和免疫细胞化学方法检测50例乳腺癌(浸润性导管癌)病变、50例乳腺良性病变(纤维腺瘤,乳腺腺病)及乳腺癌细胞系MDA-MB-468中SSEA-3和SSEA-4的表达。结果:SSEA-3在乳腺癌中的表达阳性率为34%(17/50),且SSEA-3在乳腺癌中的表达与ER表达呈正相关(P<0.05),而在乳腺良性病变中的表达阳性率为16%(8/50);SSEA-4在乳腺癌中的表达阳性率为58%(29/50),在乳腺良性病变组织中表达阳性率为36%(18/50);SSEA-3和SSEA-4在乳腺癌中的表达均高于其在良性病变中的表达(P<0.05);且两者在MDA-MB-468细胞系中均有阳性表达。结论:SSEA-3和SSEA-4在乳腺癌良恶性病变中的表达有区别,而且在乳腺癌细胞系MDA-MB-468中表达阳性率较高,提示SSEA-3和SSEA-4的表达可能与乳腺癌的发生有关。
Objective: To investigate the expression of stage-specific embryonic antigen SSEA-3 and stage-specific embryonic antigen SSEA-4 in benign and malignant breast tumors and breast cancer cell line MDA-MB-468 and its relationship with breast cancer. Methods: Fifty cases of breast cancer (invasive ductal carcinoma), 50 cases of benign breast lesions (fibroadenoma, breast adenosis) and SSEA of breast cancer cell line MDA-MB-468 were detected by immunohistochemistry and immunocytochemistry -3 and SSEA-4 expression. Results: The positive expression rate of SSEA-3 in breast cancer was 34% (17/50), and the expression of SSEA-3 in breast cancer was positively correlated with ER (P <0.05), while in benign breast lesions The positive rate of SSEA-4 expression in breast cancer was 58% (29/50), while it was 36% (18/50) in benign breast lesions. The expression of SSEA-3 and SSEA-4 in breast cancer was higher than that in benign lesions (P <0.05). Both of them were positive in MDA-MB-468 cell line. Conclusions: The expression of SSEA-3 and SSEA-4 in benign and malignant lesions of breast cancer is different, and the positive expression rate in breast cancer cell line MDA-MB-468 is higher, suggesting that the expression of SSEA-3 and SSEA-4 may be And the occurrence of breast cancer.