在pH 8.0的缓冲体系中,肝素使得罗丹明6G的荧光发生明显猝灭,罗丹明6G的荧光猝灭程度在一定范围内与肝素的浓度成正比,据此建立了荧光猝灭法测定痕量肝素的方法,并对体系的荧光猝灭机理进行了讨论。体系的激发波长为482 nm,发射波长为560 nm,线性范围为0.05~3.0 mg/L,检出限为6.1μg/L。方法已应用于肝素钠注射液中肝素含量的测定。 In the pH 8.0 buffer system, heparin quenches the fluorescence of rhodamine 6G. The fluorescence quenching of rhodamine 6G is proportional to the concentration of heparin within a certain range. Fluorescence quenching method was established for the determination of trace amounts Heparin method, and fluorescence quenching mechanism of the system are discussed. The excitation wavelength of the system is 482 nm, the emission wavelength is 560 nm, the linear range is 0.05-3.0 mg / L and the detection limit is 6.1 μg / L. The method has been applied to the determination of heparin in heparin sodium injection.