论文部分内容阅读
目的:观察基质细胞衍生因子-1(SDF-1)/趋化因子受体4(CXCR4)信号通路对喉癌Hep-2细胞的增殖作用,并探讨其作用机制。方法:体外培养Hep-2细胞,SDF-1慢病毒(4μl,1×1010病毒颗粒)和同剂量siRNA SDF-1转染Hep-2细胞6 d后,细胞分为对照组、siRNA SDF-1组和SDF-1组。MTT法检测各组Hep-2细胞增殖情况。实时荧光定量PC检测各组细胞SDF-1、CXCR4及血管内皮因子C(VEGF-C)mRNA相对表达。结果:镜下观察SDF-1组Hep-2细胞数量显著高于对照组和siRNA SDF-1组,MTT法显示SDF-1组Hep-2细胞在8 d增殖显著高于对照组和siRNA SDF-1组(P<0.05),实时荧光定量PCR结果表明SDF-1转染组SDF-1、CXCR4和VEGF-C mRNA表达显著高于对照组和siRNA SDF-1组(P<0.05),而对照组和siRNA SDF-1组SDF-1、CXCR4和VEGF-C mRNA表达未见显著差异(P>0.05)。SDF1与CXCR4及VEGF-C mRNA表达显著正相关(P<0.05)。结论:SDF-1/CXCR4信号通路能够通过上调VEGF-C表达促进喉癌Hep-2细胞增殖,SDF-1/CXCR4信号通路可成为治疗喉癌新的靶点。
OBJECTIVE: To observe the proliferation of laryngeal carcinoma Hep-2 cells by SDF-1 / CXCR4 signaling pathway and to explore its mechanism. Methods: Hep-2 cells were cultured in vitro and transfected into Hep-2 cells with SDF-1 lentivirus (4μl, 1 × 1010 virus particles) and SDF-1 cells transfected with the same dose of siRNA for 6 days. Group and SDF-1 group. MTT assay Hep-2 cells in each group proliferation. Real-time quantitative PCR was used to detect the relative expression of SDF-1, CXCR4 and VEGF-C mRNA in each group. Results: The number of Hep-2 cells in SDF-1 group was significantly higher than that in control group and SDF-1 group. MTT assay showed that the proliferation of Hep-2 cells in SDF-1 group was significantly higher than that in control group and SDF- (P <0.05). The results of real-time fluorescence quantitative PCR showed that the expression of SDF-1, CXCR4 and VEGF-C mRNA in SDF-1 transfection group was significantly higher than that in control group and siRNA SDF-1 group There was no significant difference in SDF-1, CXCR4 and VEGF-C mRNA expression between SDF-1 group and siRNA group (P> 0.05). SDF1 was positively correlated with CXCR4 and VEGF-C mRNA expression (P <0.05). Conclusion: SDF-1 / CXCR4 signaling pathway can promote the proliferation of laryngeal carcinoma Hep-2 cells by up-regulating the expression of VEGF-C. SDF-1 / CXCR4 signaling pathway may be a new therapeutic target for laryngeal cancer.