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目的探讨双氢睾酮(DHT)对快速老化小鼠(SAMP8)海马CA1区突触可塑性和N-甲基-D-天冬氨酸受体1(NMDAR1)的影响。方法 6月龄雄性SAMP8小鼠21只随机分为假手术组、去势组及去势+DHT补充治疗组,每组7只。DHT剂量为1mg/(kg.d),皮下注射21d后,通过Golgi染色观察海马CA1区顶树突树突棘的变化;免疫组织化学及图像分析检测突触素和NMDAR1表达的改变。结果 1.Golgi染色,去势组海马CA1区顶树突树突棘个数明显减少;DHT补充治疗后,树突棘个数明显增多。2.去势组海马CA1区突触素和NMDAR1的表达明显减少,平均吸光度值明显低于其他组(P<0.05)。DHT补充治疗能明显增加突触素和NMDAR1的表达。结论 DHT可调节海马CA1区突触可塑性,使树突棘密度增多。DHT对突触可塑性的影响可能与其调节锥体细胞的NMDAR1有关。
Objective To investigate the effects of dihydrotestosterone (DHT) on synaptic plasticity and N-methyl-D-aspartate receptor 1 (NMDAR1) in the hippocampal CA1 of rapidly aged mice (SAMP8). Methods Twenty-one male SAMP8 mice, 6 months old, were randomly divided into sham-operation group, castration group and castrated + DHT-supplemented group, with 7 in each group. The DHT dose was 1mg / (kg · d), and subcutaneous injection for 21 days. Golgi staining was used to observe the changes of dendritic spines in the apical dendrites of hippocampal CA1 region. Immunocytochemistry and image analysis were used to detect the expression of synaptophysin and NMDAR1. The number of dendritic spines of top dendritic spines of hippocampal CA1 area inGolgi staining and castration group was significantly reduced. After DHT supplementation, the number of dendritic spines increased significantly. The expression of synaptophysin and NMDAR1 in hippocampal CA1 area of the castrated group was significantly decreased, the average absorbance value was significantly lower than other groups (P <0.05). DHT supplementation significantly increased the expression of synaptophysin and NMDAR1. Conclusions DHT can regulate the synaptic plasticity in the hippocampal CA1 area and increase dendritic spine density. The effect of DHT on synaptic plasticity may be related to its regulation of NMDAR1 in pyramidal cells.