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目的:研究中风醒脑液对脑缺血再灌注损伤的保护作用和治疗时间窗。方法:线栓法制备大鼠大脑中动脉阻断(MCAO)局灶性脑缺血模型,缺血前给予中风醒脑液(25/60)×大鼠体重×6.25,每日1次,给药7天。末次给药1h制备MCAO模型,缺血2h,再灌注24h,评价神经功能状态、脑梗死范围;放免法测定血清白介素-8(IL-8)、肿瘤坏死因子(TNF-α)、脑源性神经营养因子(BDNF)含量;中风醒脑液的治疗时间窗研究:采用MCAO局灶性脑缺血2h,再灌注24h模型,分别在缺血开始2、3、4、5、6、12、24h再灌注并继续灌胃,每6h给药1次,再灌注24h,评价神经功能状态、脑梗死范围。结果:中风醒脑液能改善大鼠脑缺血再灌注后神经功能评分,减少脑梗死范围,降低血清I L-8、TNF-α,提高血清中BDNF的含量;在缺血后2h治疗用药,能有效改善大鼠脑缺血再灌注后神经功能评分,减轻脑水肿和减少脑梗死范围,并明显优于依达拉奉组,12h开始用药,疗效下降,24h开始用药,上述各指标有下降趋势但无统计学差异。结论:中风醒脑液对脑缺血再灌注损伤有保护作用,有效治疗时机在缺血2～6h内。
Objective: To study the protective effects of Zhongfeng Xingnao liquid on cerebral ischemia-reperfusion injury and its therapeutic time window. Methods: The model of middle cerebral artery occlusion (MCAO) induced focal cerebral ischemia in rats was established by the method of thread embolism. Before stroke, stroke was induced by Xingnao brain solution (25/60) × rat body weight × 6.25 once a day Medicine for 7 days. MCAO model was established after the last administration for 1 hour, ischemia 2 hours, reperfusion 24h, evaluation of neurological status and cerebral infarction area. The levels of serum IL-8, Neurotrophic factor (BDNF) content; stroke Xingan solution treatment time window study: MCAO focal cerebral ischemia 2h, 24h reperfusion model, respectively, at the beginning of ischemia 2,3,4,5,6,12, 24h reperfusion and continue gavage, dosing once every 6h, reperfusion 24h, evaluation of neurological status, cerebral infarction range. Results: Stroke Xingnao liquid can improve neurological function score after cerebral ischemia-reperfusion in rats, reduce the range of cerebral infarction, lower serum IL-8 and TNF-α, and increase the content of BDNF in serum. , Which can effectively improve the score of neurological function after cerebral ischemia-reperfusion in rats, reduce brain edema and reduce the range of cerebral infarction, and was significantly better than edaravone group, 12h began to medication, efficacy decreased, 24h medication, the above indicators Down trend but no statistical difference. Conclusion: Stroke Xingnao liquid has a protective effect on cerebral ischemia-reperfusion injury, the effective timing of treatment in ischemic 2 ~ 6h.