制备抗人ＩＬ－３、ＩＬ－５和ＧＭ－ＣＳＦ受体β链人工合成多肽Ｄ１、Ｄ２、Ｄ３和Ｄ４共３９株ＭｃＡｂ杂交瘤细胞系，并对其分泌ＭｃＡｂ的类与亚类、效价、特异性和上清的阻断活性进行了初步分析。ＭｃＡｂ上清阻断活性结果提示，至少部分ＭｃＡｂ可与天然的受体β链发生免疫反应，并可能有阻断ＧＭ－ＣＳＦ与β链结合作用。此外，还应用人工合成多肽片段，确定了２３株抗Ｄ１ＭｃＡｂ的大致识别表位。这些ＭｃＡｂ杂交瘤细胞系的获得为进一步研究受体β链的表达与调控、β链的结构与功能以及对于诊断和研究髓样白血病细胞的病理等均有十分重要的意义。 A total of 39 McAb hybridoma cell lines, D1, D2, D3 and D4, were synthesized and their anti-IL-3, IL-5 and GM- , Specificity and supernatant blocking activity were analyzed. McAb supernatant blocking activity results suggest that at least part of the McAb with the natural receptor β chain immune response, and may block the GM-CSF and β chain binding. In addition, the synthetic epitopes of 23 anti-D1McAbs were also determined using synthetic peptide fragments. These McAb hybridoma cell lines are of great importance for the further study of the expression and regulation of the receptor β chain, the structure and function of the β chain, and for the diagnosis and study of myeloid leukemia cells.