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以无子西瓜(CitrulluslanatusMansfeld)郑抗4号无菌苗子叶为外植体,进行了体细胞胚发生及植株再生的研究,结果表明,无菌苗应先进行3d暗培养,然后采取光照16h/d和黑暗8h/d培养3d;将子叶的叶面朝下放置于培养基上的愈伤组织诱导率高于叶面朝上的培养方式;子叶诱导胚性愈伤组织的最适培养基配方为MS+6-BA2.0mg/L+KT1.0mg/L+GA31.0mg/L;诱导需要在黑暗条件下启动,进行7d暗培养,而生长分化于光照16h/d和黑暗8h/d条件下培养7d;继代3次得到的胚性愈伤组织最多;最适生根培养基配方为1/2MS+IBA0.3mg/L。体细胞胚胎诱导率最高为25%。胚性愈伤组织经进一步培养发育为成熟的体细胞胚胎,生根成为完整植株。
The germination and somatic embryogenesis of Citrullus lanatusMansfeld No.4 seedling leaves were used as explants. The results showed that the germ-free seedlings should be cultured in darkness for 3d and then light for 16h / d and dark 8h / d for 3 days. The callus induction rate of the cotyledons placed on the medium was higher than that of the foliage upward. The optimum culture medium for embryogenic callus induction by cotyledons Was MS + 6-BA2.0mg / L + KT1.0mg / L + GA31.0mg / L; the induction needed to be started under dark conditions and dark culture was carried out for7d, while the growth was differentiated into light16h / d and darkness8h / d The embryogenic callus was obtained three times in succession. The optimum medium for rooting was 1 / 2MS + IBA0.3mg / L. Somatic embryo induction rate of up to 25%. Embryogenic callus further developed into mature somatic embryos, rooted into a complete plant.