Aim:To characterize Ca~(2+)-stimulated exocytosis in isolated mouse pancreatic βcells.Methods:An improved method was described for isolation of mouse pan-creatic β cells by collagenase P.The Ca~(2+)channel current and the membranecapacitance were examined by using the whole-cell patch clamp recordingtechnique.Results:Using depolarization and flash photolysis of caged Ca~(2+)toinduce Ca~(2+)-dependent exocytosis in β cell from KM mouse,we have explored thecharacteristics of the Ca~(2+)channel current and the relationship between Ca~(2+)signals and exocytosis.The averaged peak Ca~(2+)current measured at +20 mV was-60±6 pA(n=13).Conclusion:We characterized three kinetically different pools ofvesicles in mouse pancreatic β cells,namely an immediately releasable pool,areadily releasable pool,and a reserve pool. Aim: To characterize Ca ~ (2 +) - stimulated exocytosis in isolated mouse pancreatic β cells. Methods: An improved method was described for isolation of mouse pan-creatic β cells by collagenase P. The Ca ~ (2+) channel current and the membranecapacitance were examined by using the whole-cell patch clamp recording technique. Results: Using depolarization and flash photolysis of caged Ca ~ (2+) to nduce Ca ~ (2 +) - dependent exocytosis in β cell from KM mouse, we have explored the characteristics of the Ca ~ (2+) channel current and the relationship between Ca ~ (2+) signals and exocytosis.The averaged peak Ca ~ (2+) current measured at +20 mV was -60 ± 6 pA (n = 13). Conclusion: We characterized three kinetically different pools ofvesicles in mouse pancreatic β cells, which an immediately releasable pool, areadily releasable pool, and a reserve pool.