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目的对2016年景洪市一例鼠疫疑似患者进行判定,并对该疫情进行处置。方法对患者的血培养物进行细菌学检测、抗原检测和实时荧光PCR检测;对患者血清进行抗体检测;开展现场流行病学调查;对疫区进行消毒、灭蚤、灭鼠,开展环境卫生整治等。结果患者的血培养物细菌学检测、抗原检测、荧光PCR检测均为阳性,患者入院第12日血清抗体检测效价为1∶320;流行病学调查发现患者曾高热,有死鼠接触史,在患者居住地发现鼠间疫情;疫区经处置后,达到灭鼠灭蚤标准,无新发鼠疫患者及疑似患者。结论本次现场工作,成功判定为鼠疫,败血症型,同时建立了一套针对患者血培养物进行快速实验室诊断的流程。
Objective To determine a case of suspected plague in Jinghong City in 2016 and to dispose of the outbreak. Methods Bacteriological examination, antigen detection and real-time fluorescence PCR detection were performed on the blood cultures of patients. Antibody detection was carried out on the serum of patients. Epidemiological investigation was carried out on the patients’ blood samples. Disinfection, flea control and rodent control were carried out in the affected areas. Environmental sanitation Wait. Results The blood cultures of the patients were tested by bacteriological test, antigen test and fluorescent PCR test. The serum antibody titer was 1: 320 on the 12th day after hospital admission. Epidemiological survey found that the patients had fever, contact history of dead rats, In the patient’s place of residence found that the outbreak of the epidemic; affected area after disposal, to achieve the standard rodent killing fleas, no new plague patients and suspected patients. Conclusion The field work was successfully judged to be plague and sepsis, and a set of procedures for rapid laboratory diagnosis of blood cultures of patients was established.