Buruli溃疡是由溃疡分枝杆菌引起的 ,以皮肤深层坏死为特征的分枝杆菌病 ,常见于上、下肢。目前尚无特效疫苗 ,但有文献提示卡介苗 (BCG)有交叉保护作用。为明确BCG预防溃疡分枝杆菌感染的机理 ,作者从溃疡分枝杆菌培养物中提取 DNA进行聚合酶链反应 (PCR)扩增 ,用来自 BCG Ag8 Buruli ulcer is caused by Mycobacterium ulcerans, mycobacterial disease characterized by deep skin necrosis, common in upper and lower limbs. There is no effective vaccine, but there is literature suggests that BCG cross-protection. In order to clarify the mechanism by which BCG prevents Mycobacterium ulcerans infection, the authors extracted DNA from M. ulcerans cultures for polymerase chain reaction (PCR) amplification using primers from BCG Ag8