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导入小麦 DNA的水稻品种洛伊的三个 F4 代变异系 MD1 1 - 2、MD2 0 - 4和 MD1 8- 4,其寒、旱抗性的相关生理性状有明显差异 ,且与 DNA供体和受体 (亲本 )的明显不同 .在 4℃低温时 ,MD1 1 - 2和 MD1 8- 4和亲本细胞膜透性没明显变化 ,MD2 0 - 4的细胞膜透性则增大 ;在脱水过程中 ,三个变异系的电导率曲线的转折变化与洛伊相似 ,但其值均比它高 .在低温下 ,MD2 0 - 4和 MD1 8- 4的游离氨基酸和可溶性糖含量的变化模式基本相似 ,而与 MD1 1 - 2的变化有些不同 ;但在脱水条件下 ,三者的游离氨基酸含量变化均有差别 ;MD2 0 - 4和 MD1 8- 4的可溶性糖含量变化相似 ,且均比 MD1 1 - 2的高 .在低温下 ,三个变异系的脯氨酸含量的升降变化差别较大 ;在脱水条件下的差别较小 .结果表明 ,用导入外源 DNA改良水稻的抗寒性和抗旱性有一定效果 ,但要注意变异系之间的差异 .
The three F4 progenies of rice varieties introduced in wheat DNA, MD1 1 - 2, MD2 0 - 4 and MD1 8 - 4, showed significant differences in physiological traits related to cold and drought resistance, Receptor (parental), the cell membrane permeability of MD1 1 - 2 and MD1 8 - 4 did not change significantly and the membrane permeability of MD2 0 - 4 increased at low temperature of 4 ℃. During dehydration, The conductivity curves of the three mutant lines showed similar transitions to those of Ley, but their values were higher than those of L. The changes of free amino acids and soluble sugar contents of MD2 0-4 and MD1 8- 4 were similar at low temperature, But slightly different from those of MD1 1 - 2. However, the content of free amino acids varied with the dehydration conditions. The soluble sugar contents of MD2 0 - 4 and MD1 8 - 2 at low temperature.The variation of proline content of the three mutant lines varied greatly under the condition of dehydration.The results showed that the introduction of exogenous DNA improved the cold resistance and drought resistance of rice Have a certain effect, but pay attention to the differences between the lines of variation.