从野生大豆中获得的具有耐旱、耐冷特性的锌指转录因子GsZFP1基因,构建到以CaMV35S为启动子、E12为增强子的植物表达载体pCEOM中,以bar基因为筛选标记基因,通过农杆菌介导法将构建的植物表达载体转化苜蓿品种农菁1号的子叶节,用含0.5mg/L草丁磷的筛选培养基进行筛选,获得了70株抗性植株,用PCR检测得到20株bar基因阳性植株,将获得的转基因植株进行GsZFP1基因的RT-PCR鉴定,获得3株RT-PCR阳性植株。结果表明,GsZFP1基因在苜蓿中得到表达。 The zinc finger transcription factor GsZFP1 gene with drought tolerance and cold tolerance characteristics obtained from wild soybean was constructed into plant expression vector pCEOM with CaMV35S as promoter and E12 as enhancer. The bar gene was screened by Agrobacterium tumefaciens Mediated by the constructed plant expression vector, the cotyledonary node of Ningjing No.1 was transformed with screening medium containing 0.5mg / L glufosinate and 70 resistant plants were obtained. Twenty plants were obtained by PCR bar gene positive plants. The obtained transgenic plants were identified by RT-PCR of GsZFP1 gene, and three RT-PCR positive plants were obtained. The results showed that the GsZFP1 gene was expressed in alfalfa.