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利用SSR标记分析了30个来自广东省甘蔗主产区的甘蔗黑穗病菌菌株的遗传多样性。用19对带型较稳定且条带清晰的引物从供试菌株群体中检测到了39个多态性位点,平均每个引物得到2.05条多态性带。UPGMA聚类分析表明,在约0.74的相似系数水平,SSR标记把30个菌株划分为5个类群,类群1有22个菌株,包含了绝大部分来自粤西和粤北的菌株及全部的广州市郊菌株;在约0.8的相似系数水平,类群1可以进一步分为2个亚群体,亚群体1包含了90%的粤北菌株,亚群体2则包含了50%的粤西菌株。研究结果表明:该菌的遗传多样性与菌株的地理来源呈显著相关,而与品种间的相关性不大。
The SSR markers were used to analyze the genetic diversity of 30 M. persicae strains from Guangdong sugarcane main producing areas. Nineteen polymorphic loci were detected from 19 populations of the tested strains with 19 pairs of more stable and band-clear primers, with an average of 2.05 bands per primer. UPGMA cluster analysis showed that at the similarity coefficient level of about 0.74, SSR markers divided 30 strains into 5 groups, group 1 had 22 strains, most of the strains from western Guangdong and northern Guangdong and all of Guangzhou Subgroup 1 included 90% of North Guangdong strains, while Sub-group 2 contained 50% of Guangdong strains. At the similar coefficient level of about 0.8, Group 1 could be further divided into 2 subgroups. The results showed that the genetic diversity of the strain was significantly related to the geographical origin of the strain, but not to the variety.