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目的建立一种制备小鼠合体滋养细胞微绒毛膜(STBM)的方法。方法收集足月孕小鼠的胎盘,利用机械分离法剪碎胎盘进行差速离心,分离小鼠STBM。用Lowry法测定制备的小鼠STBM蛋白水平,用组织多肽抗原作为小鼠STBM的标记物,采用酶联免疫吸附测定(ELISA)检测STBM的水平,利用电镜观察并分析小鼠STBM的形态结构。结果实验获得的小鼠STBM制剂的蛋白水平为(0.69±0.12)mg/mL,TPA水平为(61.49±9.78)ng/mL。电镜下小鼠STBM的直径为(228.52±90.06)nm的囊泡状结构。结论制备的小鼠STBM与人STBM高度相似,可为子痫前期致病机制的探索提供在体研究的实验基础。
Objective To establish a method for preparing mouse syncytiotrophoblastic microvilli (STBM). Methods The placenta of full-term pregnant mice was collected, and the placenta was cut out by mechanical separation for differential centrifugation to separate STBM in mice. The protein level of STBM in mice was determined by Lowry method. Tissue peptide antigen was used as the marker of STBM in mice. The level of STBM was detected by enzyme - linked immunosorbent assay (ELISA). The morphological structure of STBM was observed and analyzed by electron microscope. Results The protein level of STBM in mice was (0.69 ± 0.12) mg / mL and the level of TPA was (61.49 ± 9.78) ng / mL. Under electron microscope, the diameter of STBM was (228.52 ± 90.06) nm in vesicular structure. Conclusions The prepared mouse STBM is highly similar to human STBM, and may provide experimental basis for in vivo study on the pathogenesis of preeclampsia.