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目的检测解整合素-金属蛋白酶33(ADAM33)基因单核苷酸多态性与山东汉族人群慢性阻塞性肺疾病(COPD)的关系。方法采用PCR-RFLP对363例COPD患者和372名健康人ADAM33基因T1位点多态性进行分型,对等位基因及基因型分布进行统计学分析。结果 ADAM33基因T1位点3种基因型AA、GA、GG基因型频率在病例组中分别为0.8265、0.1680和0.0055,在对照组中分别为0.8763、0.1156和0.0081,A和G等位基因型的频率在病例组中分别为0.9105和0.0895,在对照组分别为0.9341和0.0659,该位点基因型和等位基因型分布在病例组与对照组P值分别为0.117,0.090,差异均无统计学意义。结论 ADAM33基因T1位点多态性与山东汉族人群COPD发生无明显相关性。
Objective To detect the relationship between single nucleotide polymorphism of integrin - metalloproteinase 33 (ADAM33) gene and chronic obstructive pulmonary disease (COPD) in Shandong Han population. Methods PCR-RFLP was used to genotype T1 polymorphism of ADAM33 gene in 363 COPD patients and 372 healthy subjects, and the distribution of alleles and genotypes was statistically analyzed. Results The frequencies of AA, GA and GG genotypes at T1 locus were 0.8265, 0.1680 and 0.0055 in the case group and 0.8763, 0.1156 and 0.0081 in the control group, respectively. The genotype frequencies of A and G alleles The frequencies were 0.9105 and 0.0895 in the case group and 0.9341 and 0.0659 in the control group respectively. There was no significant difference in the genotype and allele distribution between the case group and the control group (0.117 and 0.090, respectively) significance. Conclusion There is no significant correlation between polymorphism at T1 locus of ADAM33 gene and COPD in Shandong Han population.