[目的]建立HPLC同时测定葛根中葛根素、大豆苷、大豆苷元含量的检测方法。[方法]HPLC色谱条件为:C18柱,流动相为甲醇-1%醋酸水溶液梯度洗脱,检测波长250 nm,流速1.0 ml/min,柱温30℃,进样量10μl。[结果]在测定范围内,葛根中葛根素、大豆苷、大豆苷元混合对照品的线性关系良好。[结论]该方法可为葛根的质量控制及综合开发提供参考。 [Objective] The research aimed to establish a HPLC method for simultaneous determination of puerarin, daidzin and daidzein in Pueraria lobata. [Method] The chromatographic conditions of HPLC were as follows: C18 column with gradient elution of methanol - 1% acetic acid as the mobile phase. The detection wavelength was 250 nm, the flow rate was 1.0 ml / min and the column temperature was 30 ℃. [Result] The linear relationship between puerarin, daidzin and daidzein in the kudzu root was good in the determination range. [Conclusion] This method can provide reference for the quality control and comprehensive development of Pueraria lobata.