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2009年至2010年,在云南省思茅地区普文镇、思茅区、震东乡、六顺乡、勐旺乡等地共采集17880只蚊子(分为91管),其中库蚊16500只(83管),按蚊1380只(8管)。采用RT-PCR方法,对91管蚊虫样本进行流行性乙型脑炎病毒NS1基因及黄热病毒E基因片段扩增,回收阳性PCR产物,并进行测序和遗传进化分析。结果表明,思茅地区库蚊乙型脑炎病毒检测阳性率为18.1%(15管/83管),黄热病毒检测阳性率为15.7%(13管/83管),思茅地区按蚊乙型脑炎病毒检测阳性率为25%(2管/8管),黄热病毒检测阳性率为12.5%(1管/8管)。克隆的流行性乙型脑炎病毒NS1基因及黄热病毒E基因与JEV和YFV参考株进行序列比对,与乙型脑炎病毒参考株同源性为88.3%～90.7%。监测结果显示,思茅地区蚊子JEV和YFV带毒率均较高,表明当地两种虫媒病毒病流行风险高。
From 2009 to 2010, a total of 17,880 mosquitoes (divided into 91 tubes) were collected in Puwen, Simao, Zhendong, Liushun and Mengwang townships in Simao Prefecture of Yunnan Province, of which 16500 were Culex (83 tubes ), Anopheles mosquito 1380 (8 tubes). Ninety-one mosquito samples were amplified by RT-PCR from epidemic areas of Japanese encephalitis virus NS1 and yellow fever virus E, and the positive PCR products were recovered and sequenced and analyzed for their genetic evolution. The results showed that the positive rate of JEV in Simao Prefecture was 18.1% (15 tubes / 83 tubes), the detection rate of yellow fever virus was 15.7% (13 tubes / 83 tubes) The positive rate of the virus was 25% (2 tubes / 8 tubes), and the positive rate of the yellow virus was 12.5% (1 tube / 8 tubes). Clone of the Japanese encephalitis virus NS1 gene and yellow fever virus E gene and JEV and YFV reference strains for sequence alignment with Japanese encephalitis virus homologous strains of 88.3% to 90.7%. Monitoring results showed that mosquitoes in Simao Prefecture had higher rates of JEV and YFV infection, indicating that there were high prevalence of two endemic arboviruses in Simao region.